Serum substitutes represent a major advancement in cell culture technology, offering defined or semi-defined solutions that overcome the limitations of animal-derived serum. Traditional fetal bovine serum (FBS) is poorly defined, variable in composition, and associated with contamination risks, ethical concerns, and supply-chain challenges. Serum replacements provide controlled formulations that improve reproducibility, enhance safety, and support consistent experimental and production outcomes.

PAN-Biotech’s Panexin product line delivers ready-to-use serum substitutes that reduce or eliminate reliance on animal serum while meeting high regulatory and GMP standards. These formulations support reliable cell growth across diverse applications, from research to industrial bioprocessing.

Adaption Protocol

For certain cells that do not tolerate a direct change from 10 % FBS-supplemented culture to 10 % Panexin we provide an easy-to-follow adaptation protocol in the respective product datasheets.

The amount of serum that is added to the culture medium is Iowered in increments of 2.5 % while the concentration of Panexin is increased by the same amount. The cells are allowed to adapt to the Iowered serum concentration for a few passages until the next adaptation step is performed. For the detachment of the cells With trypsin it is advised to use a trypsin inhibitor as Panexin itself has no trypsin-inhibiting properties.

Also check out our application note for Panexin CD which shows that the adaptation protocol can even be shortened to accelerate cell adaptation to serum-free conditions.

In some cases successful adaptation of cells to Panexin can be achieved by choosing a different basal medium.

Have questions about this products?

PAN-Biotech’s high-quality serum-free media are specifically formulated for optimized expansion of cells entirely without the need of supplementing sera. Although supplementation with serum in cell culture is common practice, it is associated with a number of disadvantages. Growing ethical concerns, a significant batch-to-batch variability and its undefined composition have led to an increasing demand on serum-free products.

Our serum-free media provide several advantages:

• highly defined cell culture conditions
• increased reproducibility of your applications
• simplified downstream and purification processes

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Stem cells are particularly interesting for academic research and the development of cell therapies.

PowerStem MSC1 is an easy-to-use xeno-free medium for in vitro proliferation of human mesenchymal stem cells (hMSC). It supports long-term growth of MSCs and keeps the cells in an undifferentiated state, which allows for specifically induced differentiation into various tissues like bone or cartilage.

In addition, MSCs cultured in PowerStem MSC1 expand faster and show a significant reduction in hematopoietic cell contamination at early passages compared to serum-based media.

Here you can find our current application note, which supports you in switching to our innovative animal and human components-free substitute Panexin.